Ainsworth struck in both halves to preserve Lytham’s 100% start to the season with a Cameron Leigh finish and an own goal completing a 4-0 win.
They are now clear leaders in the Premier Division after Vickerstown were denied three points by an injury time equaliser at Slyne-with-Hest.
Prolific marksman Scott McDonald looked to have earned the win for Dave Round’s pacesetters with two second half goals.
But Connor Crossley pulled one back for Slyne with 10 minutes left on the clock before Jamie Hastings scored from the spot in injury time to earn a 2-2 draw.
Burscough Richmond are now up to second after a 3-0 win at Coppull, Jordan Lorde grabbing a pair.
Thornton Cleveleys had the Fylde coast bragging rights with a 3-0 defeat of Blackpool Wren Rovers and Euxton Villa enjoyed the same scoreline against Southport Hesketh, Pat Bibby, Matt Atherton (pictured below) and an own goal sealing a comfortable win.
A late equaliser by Tempest's Joel Shorthouse denied Hurst Green victory which looked to be theirs following Sam Heap’s early strike.
And Turton got their first win of the season at CMB with a Joe Rodriguez penalty and Jordan Lomax strike earning the three points.
In Division One, Hesketh Bank moved to the top – for 24 hours at least – with a 2-0 win over Croston Sports.
In a game of few chances, a fine glancing header from Tom Smyth broke the deadlock early in the second half before Kevin Edgar fired home from the spot after Smyth had been tripped in the penalty box.
Askam United looked odds on to keep their own perfect record as they led 2-0 at home to Hawcoat Park.
But Frankie Ellis and Dylan Ellis had other ideas with goals to earn the visitors a point.
Stoneclough and Eagley shared six goals in a thriller with the home side coming back from a three goal half-time deficit.
Former Blackburn Rovers and Leicester professional Ben Marshall scored an 87th minute penalty to seal an unlikely point.
Lewis Hanley scored all four goals as Haslingden St Mary’s brushed aside Horwich St Mary’s who remain at the bottom without a point.
All the action at Ulverston Rangers came in the first 20 minutes as Jamie Pattinson’s strike for Millom cancelled out Matthew Lawler’s early strike.
And Jacob Carway’s effort just before the half-time whistle was enough for Kendal County to head back home along the A590 with a 1-0 win at Crooklands Casuals.
Milnthorpe Corinthians face Wyre Villa tonight, with a win enough for the visitors to return to top spot.
In Division Two, Kendal United are top with their fourth win in four games secured with goals from Bailey Kenny and James Bennett for a 2-0 win over Carnforth Rangers, who had gone into the game with their own perfect record.
Former Longridge striker Rick Coar helped Chipping to a 2-1 win over Charnock Richard Reserves while Furness Rovers are up to fifth with a Jordan Chubb-inspired win over Dalton United.
Chubb opened the scoring on five minutes before two second-half strikes sealed his hat-trick in a 4-1 win.
Latecomers to Cartmel will have been left kicking themselves as the home side and Walney Island shared six goals in a frantic first half where an Adam Harding brace helped the home side recover to earn a point in a 3-3 draw.
Ben Rothery scored twice for Storeys in a 4-1 defeat of Garstang Reserves and Galgate came away from Freckleton with a 3-2 win.
Rossendale can go top tonight with victory at Burnley United.
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